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mturquoise2 pbad  (Addgene inc)


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    Addgene inc mturquoise2 pbad
    Mturquoise2 Pbad, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mturquoise2+pbad/pmc12456950-237-13-21?v=Addgene+inc
    Average 93 stars, based on 7 article reviews
    mturquoise2 pbad - by Bioz Stars, 2026-07
    93/100 stars

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    Screen for a dual-mode FRET and BRET cAMP sensor. (a) Diagram showing how a dual-mode FRET and BRET sensor using eNL could function. Excitation can be supplied from direct excitation of FP in the eNL to operate via FRET or through the NanoLuc (NL) bioluminescence in the presence of furimazine (FZ) to operate via BRET. (b) Constructs screened for the construction of the sensor. Cyan (CeNL) and green (GeNL) eNLs incorporate an mTurqoise2 or mNeonGreen, respectively. (c, d) Dose–response curves of sensors in the HEK cell lysate in FRET (c) and BRET (d) modes (mean ± σ, n = 3). ‡ Measurements at 0 cAMP put at 10 –10 M cAMP for fitting and plotting. (e) Maximum percent change in ratio of each construct of the fits in (c, d) plotted against each other. The dashed line is y = x and data is given as mean ± 95% c.i., n = 3. Construct abbreviations (see (b) also): S, mScarlet; R, RRvT; T, tdTomato; C, CeNL (mTurquoise2-NL); G, GeNL (mNeonGreen-NL).

    Journal: ACS Omega

    Article Title: Dual-Mode FRET and BRET Sensors for Detecting cAMP Dynamics

    doi: 10.1021/acsomega.9b01770

    Figure Lengend Snippet: Screen for a dual-mode FRET and BRET cAMP sensor. (a) Diagram showing how a dual-mode FRET and BRET sensor using eNL could function. Excitation can be supplied from direct excitation of FP in the eNL to operate via FRET or through the NanoLuc (NL) bioluminescence in the presence of furimazine (FZ) to operate via BRET. (b) Constructs screened for the construction of the sensor. Cyan (CeNL) and green (GeNL) eNLs incorporate an mTurqoise2 or mNeonGreen, respectively. (c, d) Dose–response curves of sensors in the HEK cell lysate in FRET (c) and BRET (d) modes (mean ± σ, n = 3). ‡ Measurements at 0 cAMP put at 10 –10 M cAMP for fitting and plotting. (e) Maximum percent change in ratio of each construct of the fits in (c, d) plotted against each other. The dashed line is y = x and data is given as mean ± 95% c.i., n = 3. Construct abbreviations (see (b) also): S, mScarlet; R, RRvT; T, tdTomato; C, CeNL (mTurquoise2-NL); G, GeNL (mNeonGreen-NL).

    Article Snippet: HisB-RRvT (Addgene # 87364) was a generous gift from Campbell. tdTomato (Addgene #54856) and mTurquoise2 (Addgene # 54844) were generous gifts from Davidson., mNeonGreen-N1 was a generous gift from Day. pT7-CalfluxVTN (Addgene #83926) containing NanoLuc was a generous gift from Johnson.

    Techniques: Construct